Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri

Stress-related genes promote Edwardsiella ictaluri pathogenesis

Edwardsiella ictaluri is a Gram-negative facultative anaerobic rod and the causative agent of enteric septicemia of channel catfish (ESC), which is one of the most prevalent diseases of catfish, causing significant economic losses in the catfish industry. E. ictaluri is resistant to complement system and macrophage killing, which results in rapid systemic septicemia. However, mechanisms of E. i...

Molecular markers and transposon mutagenesis for genes identification

Molecular markers now help plant breeders to track useful genes conferring agronomic and quality traits, including yield, drought and heat stress resistance, insect and disease resistance, and many others. Molecular markers are simple ways to detect the genetic differences between individuals or populations. There are many types of molecular markers such as: restriction fragment length polymorp...

The Role of Edwardsiella ictaluri TonB Mutant in Edwardsiella ictaluri Virulence

Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen that causes enteric septicemia in catfish (ESC). Stress factors including poor water quality, poor diet, rough handling, overcrowding, and water temperature fluctuations increase fish susceptibility to ESC. The TonB energy transducing system (TonB-ExbB-ExbD) and TonB-dependent transporters of Gram-negative bacteria supp...

Identification and Characterization of Putative Translocated Effector Proteins of the Edwardsiella ictaluri Type III Secretion System

Edwardsiella ictaluri, a major pathogen in channel catfish aquaculture, encodes a type III secretion system (T3SS) that is essential for intracellular replication and virulence. Previous work identified three putative T3SS effectors in E. ictaluri, and in silico analysis of the E. ictaluri genome identified six additional putative effectors, all located on the chromosome outside the T3SS pathog...

Discovery, Isolation and Characterization of Bacteriophages Specific for Edwardsiella Ictaluri